کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
599077 1454261 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Atomic Force Microscopy of DNA-wrapped Single-walled Carbon Nanotubes in Aqueous Solution
ترجمه فارسی عنوان
میکروسکوپ نیروی اتمی از-DNA پیچیده تکجداره نانولوله های کربنی در محلول آبی
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
چکیده انگلیسی


• DNA-SWNT hybrids were observed in a solution.
• The same hybrid was simultaneously observed with three AFM modes.
• DNA molecules were flexible especially in a solution.

We evaluated hybrids of DNA and single-walled carbon nanotubes (SWNTs) in aqueous solution and in air using atomic force microscopy (AFM). Although intensive AFM observations of these hybrids were previously carried out for samples in air, this is the first report on AFM observations of these hybrids in solution. As expected, diameters of DNA-SWNT hybrids dramatically increased in tris(hydroxymethyl)aminomethane-ethylenediaminetetraacetic acid (TE) buffer solution. The data suggest that DNA molecules maintain their structures even on the SWNT surfaces. Furthermore, we simultaneously observed single DNA-SWNT hybrids using three different AFM modes in air and in the TE buffer solution. Height value of the hybrids was largest in the solution, and lowest for the mode that repulsive force is expected in air. For the bare SWNT molecules, height differences among the three AFM modes were much lower than those of the DNA-SWNT hybrids. DNA molecules adsorbed on SWNT surfaces flexibly changed their morphology as well as DNA molecules on flat surfaces such as mica. This is hopeful results for biological applications of DNA-SWNT hybrids. In addition, our results revealed the importance of the single-molecule approach to evaluate DNA structures on SWNT surfaces.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 143, 1 July 2016, Pages 526–531
نویسندگان
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