کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
603157 1454366 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Not only pH. Specific buffer effects in biological systems
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
Not only pH. Specific buffer effects in biological systems
چکیده انگلیسی

The aim of this work is to overview the specific effect of pH buffers in biological systems. The pH of a buffer solution changes only slightly when a small amount of a strong acid or bases is added to it. This is widely accepted and applied both in chemical and in biological (i.e. enzyme catalysis) systems. Here we show some examples – spanning from pH measurements, enzyme activities, electrophoretic mobilities, antibody aggregation, protein thermal stability – that demonstrate additional roles of buffers. They not only set pH, but also address specific ion effects, in terms of Hofmeister series, when strong electrolytes are also added. From the experimental data referred to some charged biological moieties it emerges that different buffers, at the same nominal pH, can specifically adsorb at the charged surface. Buffer specific adsorption modifies several molecular and macroscopic properties amongst which electrophoretic mobilities, and hence effective surface charges, are particularly significant. More importantly, buffers' weak electrolytes, even at low concentration, are found to compete for the adsorption at the charged surfaces with strong electrolytes, thus modulating Hofmeister effects.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Current Opinion in Colloid & Interface Science - Volume 23, June 2016, Pages 1–9
نویسندگان
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