Development and application of an analytical procedure for specific migration of green tea compounds in IV gamma nectarine active packaging

- Solid phase extraction followed by ultra phase liquid chromatography and triple quadrupole mass spectrometry.
- Specific migration studies of an active packaging containing IV gamma nectarine.
- Analysis of green tea polyphenols in real packaging.
- Analytical method to ensure the food safety and quality of IV gamma nectarine packaging.

The aim of study was to develop an easy and reliable method for the quantification of tea compounds migrated from an active packaging to the foodstuff, being IV gamma nectarine the product tested. The method was based on a solid phase extraction (SPE) followed by ultra performance liquid chromatography-mass spectrometry analysis. SPE conditions were first optimized by spiking the nectarine flesh 1% of tea extract in methanol. A previous dilution of the sample extract in water (1:10) was required to remove the major matrix interferences. Then, the washing was optimized for 10 mL water and the extraction was optimized for 0.8 mL methanol. SPE method provided recoveries ranging between 81 and 99%. Limits of detection (0.006-0.048 μg kg−1), quantification (0.008-0.160 μg kg−1) and reproducibility below to 4.2% were obtained. The method has been applied to IV gamma nectarine packaging containing green tea extract. Samples were analyzed by the methodology described and the specific migration values of tea compounds were for all the cases below to the limit of detection calculated ensuring food safety and quality of nectarine active packaging.