Isolation and characterization of protein isolated from defatted cashew nut shell: Influence of pH and NaCl on solubility and functional properties

- Protein-rich product (91.07% purity) was isolated from defatted cashew nut shell.
- The isoelectric point of defatted cashew nut shell protein was found at pH 3.
- The adjustment of pH and NaCl concentration improved the functionality of protein.
- Defatted cashew nut shell protein has comparable properties to the commercial ones.
- This protein isolate could be a potential source as food protein ingredient.

This work reports the isolation of protein from defatted cashew nut shell (CNS), with the crude protein product containing 91.07% protein. Under its natural conditions, the solubility of this protein isolate is comparable (74.02%) to that of mustard green meal protein. The solubility of the protein isolate decreases with decreasing pH, with the minimum solubility observed at its isoelectric point (pH 3). The water holding capacity, oil holding capacity, foaming capacity, foam stability, emulsifying capacity and emulsion stability were found to be 2.56 cm3 H2O/g protein, 4.28 cm3 oil/g protein, 76.88%, 70.98%, 62.0% and 79.0%, respectively. The profiles of these functional properties were determined with varying pH values and NaCl concentrations, and improved properties were observed in the alkaline pH range and in the presence of NaCl. Electrophoretic analysis showed that the high molecular weight protein globulin was the major protein in the protein isolate.