کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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6450702 | 1416136 | 2017 | 11 صفحه PDF | دانلود رایگان |
The creation of physiologically-relevant human cardiac tissue with defined cell structure and function is essential for a wide variety of therapeutic, diagnostic, and drug screening applications. Here we report a new scalable method using Faraday waves to enable rapid aggregation of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) into predefined 3D constructs. At packing densities that approximate native myocardium (108-109 cells/ml), these hiPSC-CM-derived 3D tissues demonstrate significantly improved cell viability, metabolic activity, and intercellular connection when compared to constructs with random cell distribution. Moreover, the patterned hiPSC-CMs within the constructs exhibit significantly greater levels of contractile stress, beat frequency, and contraction-relaxation rates, suggesting their improved maturation. Our results demonstrate a novel application of Faraday waves to create stem cell-derived 3D cardiac tissue that resembles the cellular architecture of a native heart tissue for diverse basic research and clinical applications.
Schematic summary of the bioengineering approach to create 3D cardiac tissue analogues. A: Culture and cardiomyocyte (CM) differentiation of human induced pluripotent stem cells (hiPSCs). B: Faraday wave patterning of hiPSC-CMs in fibrin prepolymer, generating highly-packed 3D cell construct. C: Cell encapsulation in 3D fibrin hydrogel and culture in vitro, leading to the formation of inter-connected cell bands that exhibit physiologically-relevant CM density and contractile function.Figure optionsDownload high-quality image (318 K)Download as PowerPoint slide
Journal: Biomaterials - Volume 131, July 2017, Pages 47–57