کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6450747 1416138 2017 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mitigating hypoxic stress on pancreatic islets via in situ oxygen generating biomaterial
ترجمه فارسی عنوان
تسکین استرس هیپوکسیک روی جزایر پانکراس از طریق بیومارکرهای تولید اکسیژن در محل
کلمات کلیدی
هیپوکسیا، اکسیژن، استرس بتا بیومارکرهای،
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی

A major obstacle in the survival and efficacy of tissue engineered transplants is inadequate oxygenation, whereby unsupportive oxygen tensions result in significant cellular dysfunction and death within the implant. In a previous report, we developed an innovative oxygen generating biomaterial, termed OxySite, to provide supportive in situ oxygenation to cells and prevent hypoxia-induced damage. Herein, we explored the capacity of this biomaterial to mitigate hypoxic stress in both rat and nonhuman primate pancreatic islets by decreasing cell death, supporting metabolic activity, sustaining aerobic metabolism, preserving glucose responsiveness, and decreasing the generation of inflammatory cytokines. Further, the impact of supplemental oxygenation on in vivo cell function was explored by the transplantation of islets previously co-cultured with OxySite into a diabetic rat model. Transplant outcomes revealed significant improvement in graft efficacy for OxySite-treated islets, when transplanted within an extrahepatic site. These results demonstrate the potency of the OxySite material to mitigate activation of detrimental hypoxia-induced pathways in islets during culture and highlights the importance of in situ oxygenation on resulting islet transplant outcomes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 129, June 2017, Pages 139-151
نویسندگان
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