کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6491732 | 43436 | 2013 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In silico optimization and low structured kinetic model of poly[(R)-3-hydroxybutyrate] synthesis by Cupriavidus necator DSM 545 by fed-batch cultivation on glycerol
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Glycerol was utilized by Cupriavidus necator DSM 545 for production of poly-3-hydroxybutyrate (PHB) in fed-batch fermentation. Maximal specific growth rates (0.12 and 0.3 hâ1) and maximal specific non-growth PHB production rate (0.16 g gâ1 hâ1) were determined from two experiments (inocula from exponential and stationary phase). Saturation constants for nitrogen (0.107 and 0.016 g Lâ1), glycerol (0.05 g Lâ1), non-growth related PHB synthesis (0.011 g Lâ1) and nitrogen/PHB related inhibition constant (0.405 g Lâ1), were estimated. Five relations for specific growth rate were tested using mathematical models. In silico performed optimization procedures (varied glycerol/nitrogen ratio and feeding) has resulted in a PHB content of 70.9%, shorter cultivation time (23 h) and better PHB yield (0.347 g gâ1). Initial concentration of biomass 16.8 g Lâ1 and glycerol concentration in broth between 3 and 5 g Lâ1 were decisive factors for increasing of productivity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 168, Issue 4, December 2013, Pages 625-635
Journal: Journal of Biotechnology - Volume 168, Issue 4, December 2013, Pages 625-635
نویسندگان
Ivna Vrana Å poljariÄ, Markan Lopar, Martin Koller, Alexander Muhr, Anna Salerno, Angelika Reiterer, Predrag Horvat,