کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6618812 | 1424336 | 2017 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mannheimia haemolytica growth and leukotoxin production for vaccine manufacturing - A bioprocess review
ترجمه فارسی عنوان
رشد تولیدمثلیما و تولید لکوکتوکسین برای تولید واکسن - بازنگری بیو پروسس
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
مهندسی شیمی (عمومی)
چکیده انگلیسی
Mannheimia haemolytica leukotoxin (LKT) is a known cause of bovine respiratory disease (BRD) which results in severe economic losses in the cattle industry (up to USD 1Â billion per year in the USA). Vaccines based on LKT offer the most promising measure to contain BRD outbreaks and are already commercially available. However, insufficient LKT yields, predominantly reflecting a lack of knowledge about the LKT expression process, remain a significant engineering problem and further bioprocess optimization is required to increase process efficiency. Most previous investigations have focused on LKT activity and cell growth, but neither of these parameters defines reliable criteria for the improvement of LKT yields. In this article, we review the most important process conditions and operational parameters (temperature, pH, substrate concentration, dissolved oxygen level, medium composition and the presence of metabolites) from a bioprocess engineering perspective, in order to maximize LKT yields.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electronic Journal of Biotechnology - Volume 28, July 2017, Pages 95-100
Journal: Electronic Journal of Biotechnology - Volume 28, July 2017, Pages 95-100
نویسندگان
Tobias Oppermann, Nadine Busse, Peter Czermak,