Mixotrophic cultivation of Chlorella vulgaris and its potential application for the oil accumulation from non-sugar materials

► Microalgae cultured for oil accumulation for biofuel produciton. ► C. vulgaris at autotrophic, heterotrophic and mixotrophic growth mode. ► C. vulgaris is truly mixotrophic. ► Glycerol strongly inhibits the cell growth of C. vulgaris. ► C. vulgaris utilize acetate, intermediate of anaerobic digestion.