کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
735513 1461717 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Substrate-dependent cell elasticity measured by optical tweezers indentation
ترجمه فارسی عنوان
انعطاف پذیری سلول وابسته به بستر به وسیله تیزرهای تیغه های نوری اندازه گیری می شود
موضوعات مرتبط
مهندسی و علوم پایه سایر رشته های مهندسی مهندسی برق و الکترونیک
چکیده انگلیسی


• We propose a simple approach to locally measure cell elasticity by cell indentation.
• We applied pN forces at low loading rates in axial direction.
• Cell–bead interaction can be studied in all the three directions.
• We measured elastic modulus of HBL-100 cells.
• We demonstrated the cell׳s stiffness dependence on the substrate.

In the last decade, cell elasticity has been widely investigated as a potential label free indicator for cellular alteration in different diseases, cancer included. Cell elasticity can be locally measured by pulling membrane tethers, stretching or indenting the cell using optical tweezers. In this paper, we propose a simple approach to perform cell indentation at pN forces by axially moving the cell against a trapped microbead. The elastic modulus is calculated using the Hertz-model. Besides the axial component, the setup also allows us to examine the lateral cell–bead interaction. This technique has been applied to measure the local elasticity of HBL-100 cells, an immortalized human cell line, originally derived from the milk of a woman with no evidence of breast cancer lesions. In addition, we have studied the influence of substrate stiffness on cell elasticity by performing experiments on cells cultured on two substrates, bare and collagen-coated, having different stiffness. The mean value of the cell elastic modulus measured during indentation was 26±9 Pa for the bare substrate, while for the collagen-coated substrate it diminished to 19±7 Pa. The same trend was obtained for the elastic modulus measured during the retraction of the cell: 23±10 Pa and 13±7 Pa, respectively. These results show the cells adapt their stiffness to that of the substrate and demonstrate the potential of this setup for low-force probing of modifications to cell mechanics induced by the surrounding environment (e.g. extracellular matrix or other cells).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Optics and Lasers in Engineering - Volume 76, January 2016, Pages 27–33
نویسندگان
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