کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7614819 | 1493977 | 2018 | 30 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Validation of analytical methods for chlordecone and its metabolites in the urine and feces of ewes
ترجمه فارسی عنوان
اعتبار سنجی روش های تحلیلی برای کلردکون و متابولیت های آن در ادرار و مدفوع جوجه
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
To be able to monitor the fate of chlordecone and its metabolites in livestock and to assess if the compounds could be released in the environment, urinary and fecal samples were logically targeted. In order to reach this goal, robust and validated analytical methods are required. For this purpose, Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) extraction methods were validated to analyze CLD and its metabolites in the urine and feces. The analysis was carried using liquid phase chromatography with tandem mass spectrometry and validated according to French standard NF V03-110 and SANTE guidelines. Matrix effect, Accuracy, within-laboratory repeatability, specificity, Q/q relative ion intensities and uncertainty were reported. Recoveries between 70% and 120% were obtained from urine and feces. The limits of quantification (LOQ) in urine samples were 0.1â¯Î¼gâ¯CLDâ¯Lâ1, 0.1â¯Î¼gâ¯total CLD (CLD and its conjugates)·Lâ1, 1.3â¯Î¼gâ¯CLDOHâ¯Lâ1 and 2.4â¯Î¼gâ¯total CLD (chlordecol and its conjugates)â¯Lâ1 of urine. LOQ in fresh feces were 3.2â¯Î¼gâ¯CLDâ¯kgâ1 and 5.8â¯Î¼gâ¯CLDOHâ¯kgâ1. Contaminated urinary and fecal samples from ewes were analyzed to confirm the relevance of the methods. In urine, CLD and conjugated CLDOH could be quantified whereas only free CLD and free CLDOH were found in feces. These methods are essential for future toxicokinetic studies and also to estimate the environmental contamination.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volumes 1093â1094, 1 September 2018, Pages 66-76
Journal: Journal of Chromatography B - Volumes 1093â1094, 1 September 2018, Pages 66-76
نویسندگان
Maïlie Saint-Hilaire, Thomas Bertin, Chanthadary Inthavong, Gwenaëlle Lavison-Bompard, Thierry Guérin, Agnès Fournier, Cyril Feidt, Guido Rychen, Julien Parinet,