کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7626979 | 1494583 | 2018 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development and validation of UPLC-MS/MS assay for quantification of cladrin: Absolute bioavailability and dose proportionality study in rats
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
Cladrin, an isoflavone is a major bioactive constituent found in stem bark of Butea monosperma with remarkable osteogenic activity. A speedy and sensitive UPLC coupled tandem mass spectrometry (UPLC-MS/MS) method was developed, validated and successfully applied to bioavailability, blood partitioning, plasma protein binding, intravenous and multiple-dose oral pharmacokinetics of cladrin in rats. Separation was done on C18 column (5.0â¯Î¼m, 4.6â¯Ãâ¯50â¯mm) using mobile phase containing acetonitrile and 0.10% formic acid in the ratio of 65:35 (v/v) with 0.60â¯mL/min flow rate. The method was highly sensitive and has a short run time of 2.50â¯min with an excellent linearity (R2â¯>â¯0.99) in the range of 0.20-200â¯Î¼g/L. Absolute bioavailability was found to be 16.58, 19.04 and 6.76% at oral doses of 5, 10, and 20â¯mg/Kg, respectively. Cladrin was rapidly absorbed (Tmax 3.0â¯h) with a high apparent volume of distribution (15.03â¯Â±â¯1.79L/Kg), high clearance (2.27â¯Â±â¯0.30L/h/Kg) and high plasma protein binding. The present study is a first comprehensive in-vitro as well as the in-vivo preclinical pharmacokinetic report of cladrin giving insights about its drug-likeness and further development as a potential therapeutic agent.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 152, 15 April 2018, Pages 289-297
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 152, 15 April 2018, Pages 289-297
نویسندگان
Mamunur Rashid, Sandeep K. Singh, Mohd Yaseen Malik, Sadaf Jahan, Swati Chaturvedi, Isha Taneja, Kanumuri Sivarama Raju, Zaiba Naseem, J.R. Gayen, Muhammad Wahajuddin,