Production of dry extract extracellular lipase from Aspergillus niger by solid state fermentation method to catalyze biodiesel synthesis

Lipase can be produced from Aspergillus niger using solid state fermentation method. This research aimed to produce lipase in powder form. The highest activity unit (38.67 U/g) was obtained from rice bran substrate at 5th day with inducer concentration of 2%. The best drying method to produce dry extract with activity unit of 566.67 U/g was freeze drying with maltodextrin additive. The best drying method to produce fine powder with activity unit of 333.33 U/g was spray drying with skim powder additive. Supernatant and powder lipase worked optimally at 30 °C with activity unit of 44.00 U/g and 355.56 U/g respectively.