کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7986879 1515163 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Atomic force microscopy observation of lipopolysaccharide-induced cardiomyocyte cytoskeleton reorganization
ترجمه فارسی عنوان
مشاهده میکروسکوپ نیروی اتمی از ترمیم سیتواسکلت قلب بیمار مبتلا به لیپوپلی ساکارید
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد دانش مواد (عمومی)
چکیده انگلیسی
We applied atomic force microscopy (AFM) to observe lipopolysaccharide (LPS)-induced intracellular cytoskeleton reorganization in primary cardiomyocytes from neonatal mouse. The nonionic detergent Triton X-100 was used to remove the membrane, soluble proteins, and organelles from the cell. The remaining cytoskeleton can then be directly visualized by AFM. Using three-dimensional technique of AFM, we were able to quantify the changes of cytoskeleton by the “density” and total “volume” of the cytoskeleton fibers. Compared to the control group, the density of cytoskeleton was remarkably decreased and the volume of cytoskeleton was significantly increased after LPS treatment, which suggests that LPS may induce the cytoskeleton reorganization and change the cardiomyocyte morphology.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Micron - Volume 51, August 2013, Pages 48-53
نویسندگان
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