High resolution imaging of subcellular glutathione concentrations by quantitative immunoelectron microscopy in different leaf areas of Arabidopsis

► Determination of subcellular glutathione concentrations in different Arabidopsis leaf areas. ► Measurement of cell volumes and glutathione gold particle density by TEM. ► Glutathione concentrations of up to 15 mM were calculated for mitochondria. ► Main differences of glutathione contents between the leaf areas in vacuoles and mitochondria.