کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8296722 | 1536770 | 2015 | 31 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
High fructose consumption induces DNA methylation at PPARα and CPT1A promoter regions in the rat liver
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کلمات کلیدی
LDLRPPARαMTTPDyslipidemia - اختلالات چربی خون یا دیسپیدمیEpigenetics - اپی ژنتیکGene expression - بیان ژنNutrition - تغذیهMetabolic syndrome - سندرم متابولیکCorn syrup - شربت ذرتmicrosomal triglyceride transfer protein - پروتئین انتقال تری گلیسرید میکروسمالیcarnitine palmitoyltransferase 1a - کارنتین پالمیتیل ترانسفراز 1alow density lipoprotein receptor - گیرنده لیپوپروتئین چگالی کمperoxisome proliferator-activated receptor α - گیرنده پروتئینی فعال پروکسایزوم α
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
DNA methylation status is affected by environmental factors, including nutrition. Fructose consumption is considered a risk factor for the conditions that make up metabolic syndrome such as dyslipidemia. However, the pathogenetic mechanism by which fructose consumption leads to metabolic syndrome is unclear. Based on observations that epigenetic modifications are closely related to induction of metabolic syndrome, we hypothesized that fructose-induced metabolic syndrome is caused by epigenetic alterations. Male SD rats were designated to receive water or 20% fructose solution for 14 weeks. mRNA levels for peroxisome proliferator-activated receptor alpha (PPARα) and carnitine palmitoyltransferase 1A (CPT1A) was analyzed using Real-time PCR. Restriction digestion and real-time PCR (qAMP) was used for the analysis of DNA methylation status. Hepatic lipid accumulation was also observed by fructose intake. Fructose feeding also significantly decreased mRNA levels for PPARα and CPT1A. qAMP analysis demonstrated the hypermethylation of promoter regions of PPARα and CTP1A genes. Fructose-mediated attenuated gene expression may be mediated by alterations of DNA methylation status, and pathogenesis of metabolic syndrome induced by fructose relates to DNA methylation status.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 468, Issues 1â2, 4â11 December 2015, Pages 185-189
Journal: Biochemical and Biophysical Research Communications - Volume 468, Issues 1â2, 4â11 December 2015, Pages 185-189
نویسندگان
Koji Ohashi, Eiji Munetsuna, Hiroya Yamada, Yoshitaka Ando, Mirai Yamazaki, Nao Taromaru, Ayuri Nagura, Hiroaki Ishikawa, Koji Suzuki, Ryoji Teradaira, Shuji Hashimoto,