کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8325533 | 1539946 | 2010 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Scleraxis and E47 cooperatively regulate the Sox9-dependent transcription
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
E47G3PDHFGFCol2a1TGFP300Sox9bHLHSmall interfering RNA - RNA تداخل کوچکsiRNA - siRNAElectrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزbasic helix-loop-helix - اسلحه پایه حلقه ایscx - اسکاScleraxis - اسکلراکسیسtransforming growth factor - تبدیل فاکتور رشدImmunoprecipitation - تخریب ایمنیactivation domain - دامنه فعال سازیTranscription - رونویسیEMSA یا electrophoretic mobility shift assay - سنجش تغییر تحرک الکتروفورتیکfibroblast growth factor - فاکتور رشد فیبروبلاستBMP - مدیریت فرایند کسب و کارWestern blot - وسترن بلاتBone morphogenetic protein - پروتئین مورفوژنیک استخوانglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
During musculoskeletal development, Sry-type HMG box 9 (Sox9) has a crucial role in mesenchymal condensation and chondrogenesis. On the other hand, a tissue-specific basic helix-loop-helix (bHLH) transcription factor Scleraxis (Scx) regulates the differentiation of tendon and ligament progenitors. Whereas these two transcription factors cooperatively participate in the determination of cellular lineages, the precise interaction between Sox9 and Scx remains unclear. We have previously demonstrated that the Sox9-dependent transcription is synergistically activated by several Sox9-associating molecules, such as p300 and Smad3, on chromatin. In this study, we investigated the function of Scx in the Sox9-dependent transcription. The expression of α1(II) collagen (Col2a1) gene was stimulated by an appropriate transduction of Sox9 and Scx. Scx and its partner E47, which dimerizes with other bHLH proteins, cooperatively enhanced the Sox9-dependent transcription in luciferase reporter assays. Coactivator p300 synergistically increased the activity of Sox9-regulated reporter gene, which contains promoter and enhancer regions of Col2a1, in the presence of Scx and E47. Immunoprecipitation analyses revealed that Scx and E47 formed a transcriptional complex with Sox9 and p300. Scx/E47 heterodimer also associated with a conserved E-box sequence (CAGGTG) in the Col2a1 promoter on chromatin. These findings suggest that Scx and E47 might modulate the primary chondrogenesis by associating with the Sox9-related transcriptional complex, and by binding to the conserved E-box on Col2a1 promoter.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 42, Issue 1, January 2010, Pages 148-156
Journal: The International Journal of Biochemistry & Cell Biology - Volume 42, Issue 1, January 2010, Pages 148-156
نویسندگان
Takayuki Furumatsu, Chisa Shukunami, Michiyo Amemiya-Kudo, Hitoshi Shimano, Toshifumi Ozaki,