کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8386826 | 1543854 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
SPL7 locally regulates copper-homeostasis-related genes in Arabidopsis
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کلمات کلیدی
GFPACT2PYEGUSqRT-PCRROS - ROSβ-glucuronidase - β-گلوکورونیدازArabidopsis thaliana - آرابیدوپسیس تالیاناIron - آهنquantitative reverse transcription PCR - رونویسی معکوس PCR کمیIron superoxide dismutase - سوپر اکسید دیسموتاز آهنICP-MS - طیفسنجی جرمی پلاسمای جفتشده القاییinductively coupled plasma mass spectrometry - طیفسنجی جرمی پلاسمای جفتشده القاییZinc - فلز رویCopper - مسMicroRNA - میکرو RNA MiRNA - میکروRNA، ریزآرانای، miRNAwild-type - نوع وحشیSignal transduction - هدایت سیگنالgreen fluorescent protein - پروتئین فلورسنت سبزGrafting - پیوند زدنCopper deficiency - کمبود مسReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In Arabidopsis, a central regulator of copper (Cu) homeostasis is the transcription factor SQUAMOSA promoter binding protein-like7 (SPL7). Under Cu deficiency, SPL7 induces the expression of miR398, which suppresses the expression of the genes CSD1 and CSD2, which encode cytosolic and chloroplastic isoforms of Cu/Zn superoxide dismutase, respectively. Consequently, the limited Cu is preferentially assigned to plastocyanin, which is essential for photosynthetic electron transport. Consistent with this function of miR398 related to photosynthesis, its expression is strongly induced in leaves. In this study, however, we showed that SPL7 was transcribed mainly around the vasculature in roots, where Cu levels were likely sensed. To test the possible long-distance signaling of Cu availability from roots to shoots, we conducted a series of grafting experiments using spl7 mutant and wild-type (WT) plants. Expression of Cu-responsive microRNAs and the resulting suppression of CSD1 and CSD2 mRNAs were observed in leaves only when the aerial part was from WT plants, in which a low level of SPL7 was transcribed also in the vascular tissues. Although local sensing of Cu was disturbed in the spl7 mutant, the Cu level was not affected in the shoots. SPL7 is expressed in specific cell layers in both roots and shoots and locally senses Cu availability, transmitting the information to surrounding cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Plant Physiology - Volumes 224â225, MayâJune 2018, Pages 137-143
Journal: Journal of Plant Physiology - Volumes 224â225, MayâJune 2018, Pages 137-143
نویسندگان
Ryoichi Araki, Mélanie Mermod, Hiroaki Yamasaki, Takehiro Kamiya, Toru Fujiwara, Toshiharu Shikanai,