کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8393198 | 1544059 | 2017 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
MicroRNA-27a-3p affects estradiol and androgen imbalance by targeting Creb1 in the granulosa cells in mouse polycytic ovary syndrome model
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
CCK-8PCNACYP19A1qPCRmGCGAPDHPNACDK4CDK2P450sccCREB1snRNADHTCdk6 - cdk6small nuclear RNA - RNA هسته ای کوچکProliferating Cell Nuclear Antigen - آنتیژن هسته ای تکثیر سلولیEstradiol - استرادیولDihydrotestosterone - دی هیدروتستوسترونStar - ستارهgranulosa cell - سلول گرانولوزاcyclin-dependent kinase 2 - سیکلین وابسته به کیناز 2cyclin-dependent kinase 6 - سیکلین وابسته به کیناز 6cell counting kit-8 - شمارش سلول کیت 8MicroRNA - میکرو RNA MiRNA - میکروRNA، ریزآرانای، miRNAquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیSteroidogenic acute regulatory protein - پروتئین حاکم استروئیدوژنیک حادPropidium iodide - پروتئین یدیدProgesterone - پروژسترونcyclin-dependent kinase 4 - کییناز وابسته به سیکلین 4glyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Polycystic ovary syndrome (PCOS) is a common endocrine abnormality in women characterized by a menstrual disturbance with chronic anovulation and hyperandrogenism, polycystic ovaries, and insulin resistance. MicroRNAs (miRNAs) are important fine-tune regulators involved in various physiological and pathological processes, but their actions are not fully understood. In this study, we observed the increased expression of miR-27a-3p in the ovaries of mice with PCOS and explored its functions in primary mouse granulosa cells (mGCs) and the mouse granulosa-like tumor cell line, KK-1, using several approaches. QPCR results showed that miR-27a-3p expression was significantly higher in mGCs at the preantral follicle (PrF) stage. Using flow cytometry and hormone analysis, we found that overexpression of miR-27a-3p promoted apoptosis and inhibited estradiol (E2) production in KK-1 cells. Moreover using a luciferase assay and Western blotting analysis, we verified that the gene of cyclic AMP response element (CRE)-binding protein 1 (Creb1) was a potential target of miR-27a-3p, which in effect hindered the expression of its downstream factor cytochrome P450 family 19 subfamily A polypeptide 1 (Cyp19a1). With the decrease of aromatase activity, testosterone (T) is reduced to dihydrotestosterone (DHT) and this exerts its effect of upregulation of the miR-27a-3p expression. The imbalance of androgen and E2 levels affected by miR-27a-3p and its function of promoting GC apoptosis could be involved in the pathophysiology of PCOS. Our results indicate that miR-27a-3p in PCOS GCs may play an important role in ovarian follicular development and provide new insights into GC dysfunction in PCOS.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reproductive Biology - Volume 17, Issue 4, December 2017, Pages 295-304
Journal: Reproductive Biology - Volume 17, Issue 4, December 2017, Pages 295-304
نویسندگان
Mingming Wang, Mei Liu, Jing Sun, Lina Jia, Shuoqian Ma, Jun Gao, Yunyun Xu, Hongwen Zhang, Suk Ying Tsang, Xiangdong Li,