کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8485207 | 1551699 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mycobacterium tuberculosis infection induces IL-10 gene expression by disturbing histone deacetylase 6 and histonedeacetylase 11 equilibrium in macrophages
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کلمات کلیدی
qRT-PCRIL-10MTBchromatin immunoprecipitation - ایمن سازی کروماتینInterleukin-10 - اینترلوکین 10ELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاMacrophage - ماکروفاژ Mycobacterium tuberculosis - مایکوباکتریوم توبرکلوزیسHistone deacetylase 6 - هیستون دیازتیلاز 6quantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیCHiP - چیپ
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروبیولوژی و بیوتکنولوژی کاربردی
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چکیده انگلیسی
Mycobacterium tuberculosis (MTB) infection is a significant contributor to dysregulated T cell-mediated immune response. Here we aimed to evaluate the mechanism of MTB infection in promoting interleukin-10 (IL-10) upregulation. The IL-10 levels in MTB infected THP-1Â cells were evaluated with enzyme-linked immunosorbent assay (ELISA) and quantitative real-time PCR (qRT-PCR). In challenged THP-1Â cells, the HDAC6 and HDAC11 mRNA and protein levels were monitored at varied duration after MTB infection. Further, chromatin immunoprecipitation (ChIP) analysis was used to investigate the interaction between IL-10 expression and HDAC6 or HDAC11. HDAC6 and HDAC11 were overexpressed or silenced to study their effects on IL-10 regulation. IL-10 was upregulated after MTB challenge in a time- and dose-dependent manner. Similarly, HDAC6 and HDAC11 were also upregulated by MTB challenge. Overexpression or silencing of HDAC6 and HDAC11 changed IL-10 expression correspondingly. MTB infection disturbs the HDAC6/HDAC11 levels to induce IL-10 expression. Strategies to counteract the dysregulation of HDAC6/HDAC11 would potentially alleviate the immunological disordered following MTB infection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Tuberculosis - Volume 108, January 2018, Pages 118-123
Journal: Tuberculosis - Volume 108, January 2018, Pages 118-123
نویسندگان
Xiaolei Wang, Yanhua Wu, Jin Jiao, Qing Huang,