کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
870573 1471024 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Validation of three viable-cell counting methods: Manual, semi-automated, and automated
ترجمه فارسی عنوان
اعتبار سنجی از سه روش شمارش سلول های قابل قبول: دستی، نیمه اتوماتیک، و خودکار
کلمات کلیدی
محرومیت آبی تیپان، زنده ماندن سلولها، تعداد سلول
موضوعات مرتبط
مهندسی و علوم پایه مهندسی انرژی انرژی های تجدید پذیر، توسعه پایدار و محیط زیست
چکیده انگلیسی

A viable cell count is essential to evaluate the kinetics of cell growth. Since the hemocytometer was first used for counting blood cells, several variants of the methodology have been developed towards reducing the time of analysis and improving accuracy through automation of both sample preparation and counting. The successful implementation of automated techniques relies in the adjustment of cell staining, image display parameters and cell morphology to obtain equivalent precision, accuracy and linearity with respect to the hemocytometer. In this study we conducted the validation of three trypan blue exclusion-based methods: manual, semi-automated, and fully automated; which were used for the estimation of density and viability of cells employed for the biosynthesis and bioassays of recombinant proteins. Our results showed that the evaluated attributes remained within the same range for the automated methods with respect to the manual, providing an efficient alternative for analyzing a huge number of samples.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biotechnology Reports - Volume 7, September 2015, Pages 9–16
نویسندگان
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