کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8841805 | 1615033 | 2018 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Pathological histone acetylation in Parkinson's disease: Neuroprotection and inhibition of microglial activation through SIRT 2 inhibition
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کلمات کلیدی
LPSIL6Bcl2GDNFcox2iNOSHDACLCMBDNF - BDNF یا فاکتور نورونزایی مشتقشده از مغز IL1β - IL1binterleukin 1β - اینترلوکین 1βinterleukin 6 - اینترلوکین 6Parkinson’s disease - بیماری پارکینسونinducible nitric oxide synthase - سنتاز اکسید نیتریک القاییcyclooxygenase 2 - سیکلوکوکسیژناز 2brain derived neurotrophic factor - عامل مغز استخوان مغز استخوان استGlial derived neurotrophic factor - فاکتور نوروترفیک گلیال مشتق شده استB-cell lymphoma 2 - لنفوم سلول B 2laser capture microdissection - لیزر ضبط میکرو دیسکسیونlipopolysaccharide - لیپوپلی ساکاریدNitric oxide - نیتریک اکسیدHistone acetyltransferase - هیستون استیل ترانسفرازhistone deacetylase - هیستون داستیلازHAT - کلاه
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
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چکیده انگلیسی
Parkinson's disease (PD) is associated with degeneration of nigrostriatal neurons due to intracytoplasmic inclusions composed predominantly of a synaptic protein called α-synuclein. Accumulations of α-synuclein are thought to 'mask' acetylation sites on histone proteins, inhibiting the action of histone acetyltransferase (HAT) enzymes in their equilibrium with histone deacetylases (HDACs), thus deregulating the dynamic control of gene transcription. It is therefore hypothesised that the misbalance in the actions of HATs/HDACs in neurodegeneration can be rectified with the use of HDAC inhibitors, limiting the deregulation of transcription and aiding neuronal homeostasis and neuroprotection in disorders such as PD. Here we quantify histone acetylation in the Substantia Nigra pars compacta (SNpc) in the brains of control, early and late stage PD cases to determine if histone acetylation is a function of disease progression. PD development is associated with Braak-dependent increases in histone acetylation. Concurrently, we show that as expected disease progression is associated with reduced markers of dopaminergic neurons and increased markers of activated microglia. We go on to demonstrate that in vitro, degenerating dopaminergic neurons exhibit histone hypoacetylation whereas activated microglia exhibit histone hyperacetylation. This suggests that the disease-dependent increase in histone acetylation observed in human PD cases is likely a combination of the contributions of both degenerating dopaminergic neurons and infiltrating activated microglia. The HDAC SIRT 2 has become increasingly implicated as a novel target for mediation of neuroprotection in PD: the neuronal and microglial specific effects of its inhibition however remain unclear. We demonstrate that SIRT 2 expression in the SNpc of PD brains remains relatively unchanged from controls and that SIRT 2 inhibition, via AGK2 treatment of neuronal and microglial cultures, results in neuroprotection of dopaminergic neurons and reduced activation of microglial cells. Taken together, here we demonstrate that histone acetylation is disease-dependently altered in PD, likely due the effects of dopaminergic neurodegeneration and microglial infiltration; yet SIRT 2 remains relatively unaltered with disease. Given the stable nature of SIRT 2 expression with disease and the effects of SIRT 2 inhibitor treatment on degenerating dopaminergic neurons and activated microglia detected in vitro, SIRT 2 inhibitors warrant further investigation as potential therapeutics for the treatment of the PD.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 666, 14 February 2018, Pages 48-57
Journal: Neuroscience Letters - Volume 666, 14 February 2018, Pages 48-57
نویسندگان
Ian F. Harrison, Andrew D. Smith, David T. Dexter,