کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9038408 | 1133826 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inhibitory effect of Buthus martensi Karsch extracts on interleukin-1β-induced expression of nitric oxide (NO) synthase and production of NO in human chondrocytes and LPS-induced NO and prostaglandin E2 production in mouse peritoneal macrophages
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کلمات کلیدی
NF-κB(IL-1β)TBSTAP-1nNOSIL-1βInducible NO synthase (iNOS)PGE2iNOSCOX-1Cyclooxygenase-2 (COX-2)Cyclooxygenase-1 (COX-1)COX-2HRPTBSButhus martensi KarschEMSALPSecNOSinducible NO synthase - NO سنتاز القاء شدهneuronal NOS - NOS عصبیElectrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزOsteoarthritis - استئوآرتریت(آرتروز)Interleukin-1β - اینترلوکین-1βBmk - بروTris-buffered saline - تریس بافر شورCytokine - سیتوکینcyclooxygenase-1 - سیکلوکوکسیژناز 1Cyclooxygenase-2 - سیکلوکوکسیژناز2nuclear factor-κB - فاکتور هسته ای κBlipopolysaccharide - لیپوپلی ساکاریدMacrophage - ماکروفاژ Nitric oxide - نیتریک اکسیدNitric oxide (NO) - نیتریک اکسید (NO)Horseradish peroxidase - پراکسیداز هوررادیشactivation protein-1 - پروتئین فعال سازی 1Prostaglandin E2 - پروستاگلاندین E2Prostaglandin E2 (PGE2) - پروستاگلاندین E2 (PGE2)Chondrocytes - کندروسیت
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We examined the effect of Buthus martensi Karsch (BMK) extract on IL-1β-induced production of nitrogen oxide (NO) in primary human osteoarthritis (OA) chondrocytes. The cells were treated with BMK (10 μg/ml) and IL-1β (2 ng/ml) for different periods, and inducible nitric oxide synthase (iNOS) mRNA and protein expression were determined by real-time quantitative reverse transcriptase-polymerase chain reaction and Western blotting, respectively. The cytotoxicity of BMK on human OA chondrocytes was very low (IC50 > 250 μg/ml) as measured by the XTT assay method. Production of NO was determined as nitrite in culture supernatant. Human chondrocytes cotreated with BMK produced significantly less NO compared with chondrocytes stimulated with IL-1β alone. Activation and translocation of and NF-κB DNA binding activity were determined by Western blotting and specific enzyme-linked immunosorbent assay. The inhibition of NO production correlated with the suppression of induction and expression of nuclear factor-κB (NF-κB) and activation protein-1 (AP-1)-dependent gene. BMK inhibited the activation and translocation of NF-κB to the nucleus, indicating that BMK inhibits the IL-1β-induced production of NO in human chondrocytes by interfering with the activation of NF-κB through a novel mechanism. In addition, BMK reduced prostaglandin E2 (PGE2) production in mouse peritoneal macrophages stimulated with lipopolysaccharide, whereas no influence on the activity of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2) or cyclooxygenase-1 (COX-1) was observed. Our data, therefore, suggest that BMK may be a therapeutically effective inhibitor of IL-1β-induced inflammatory effects that are dependent on NF-κB activation in human OA chondrocytes. The results indicate that BMK exerts anti-inflammatory effects related to the inhibition of neutrophil functions and of NO and PGE2 production, which could be due to a decreased expression of iNOS and COX-2 through the transcription factors NF-κB and AP-1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 19, Issue 6, September 2005, Pages 757-769
Journal: Toxicology in Vitro - Volume 19, Issue 6, September 2005, Pages 757-769
نویسندگان
Kap-Sung Kim, Hyun-Seok Cho, Seung-Deok Lee, Kyung-Ho Kim, Jae-Yong Cho, Kang-Hyun Chung, Young-Choon Lee, Sung-Kwon Moon, Cheorl-Ho Kim,