کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
95243 160422 2015 10 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله ISI
Structural analysis of quazepam metabolites in bile by ion trap time-of-flight mass spectrometry
ترجمه فارسی عنوان
تجزیه و تحلیل ساختاری متابولیت های کوئیزپام در صفراوی با استفاده از طیف سنجی جرمی زمان پرواز تله یون
کلمات کلیدی
کوازپام؛ متابولیت؛ کروماتوگرافی مایع؛ تله یونی؛ طیف سنجی جرمی زمان پرواز
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• Novel quazepam metabolites in bile are searched by LC-IT-TOF MS.
• Metabolites having a fluoro(hydroxy)phenyl group were found.
• Metabolites having a fluoro(hydroxy)(methoxy)phenyl group were found.
• A novel metabolism pathway of quazepam was postulated.

Quazepam (QZP) is a long-acting benzodiazepine-type hypnotic. We searched for novel QZP metabolites in bile and determined their structures by liquid chromatography-ion trap time-of-flight mass spectrometry (LC-IT-TOF MS). The metabolites were extracted with ethyl acetate after β-glucuronidase treatment. First, a single MS spectrum was acquired. Second, MSn spectra were acquired for peaks that consisted of ions with the isotope pattern corresponding to molecules bearing one chlorine atom. The novel QZP metabolites found in this study were hydroxyquazepam, hydroxy-methoxyquazepam, hydroxy-oxoquazepam, and hydroxy-methoxy-oxoquazepam, which have the hydroxy and methoxy groups on the fluorophenyl group, and dihydroxy-oxoquazepam and dihydroxy-methoxy-oxoquazepam, which have one hydroxy group at the 3-position of the seven-membered ring and the other hydroxy group and the methoxy group on the fluorophenyl group. We demonstrated that LC-IT-TOF MS was a useful tool for determining the structure of the metabolites. However, the exact locations of the hydroxy and methoxy groups on the fluorophenyl group could not be identified.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Forensic Science International - Volume 256, November 2015, Pages 7–16
نویسندگان
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