کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9587297 | 1393231 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
1H-13C polarization transfer in membranes: A tool for probing lipid dynamics and the effect of cholesterol
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Phospholipid bilayers with over 20% cholesterol can form a liquid-ordered (lo) phase, which can be found in lateral domains, called rafts, in biomembranes. We show here that high-resolution 13C and 1H solid-state NMR are well suited to explore this phase, intermediate between gel and fluid. This approach can be applied to artificial or natural membranes, with no isotopic enrichment and with the help of magic-angle spinning (MAS), taking advantage of the high resolution and sensitivity of these nuclei. The sensitivity of magnetization transfer schemes to different lipid states has allowed us here to discriminate between various phases. We show that the phase composed of unsaturated phospholipids and cholesterol differs, in terms of lipid dynamics, both from the previously described lo phase and from the liquid-disordered phase.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Magnetic Resonance - Volume 177, Issue 1, November 2005, Pages 166-171
Journal: Journal of Magnetic Resonance - Volume 177, Issue 1, November 2005, Pages 166-171
نویسندگان
Dror E. Warschawski, Philippe F. Devaux,