کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9894100 | 1542261 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Direct coupling between arachidonic acid-induced Ca2+ release and Ca2+ entry in HEK293 cells
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
CCEIsotetrandrineETYA2-APBIP3RThapsigarginRyRGd3+ - Gd3 +[Ca2+]i - [Ca2 +] iCa2+ release - آزادی Ca2 +5,8,11,14-eicosatetraynoic acid - اسید 5،8،11،14-ایکوزاتتریائیکArachidonic acid - اسید آراشیدونیکCoupling - جفتHEK293 cells - سلول های HEK293human embryonic kidney cells - سلول های کلیوی جنینی انسانCa2+ entry - ورودی Ca2 +Capacitative Ca2+ entry - ورودی Capacitive Ca2 +inositol 1,4,5-trisphosphate receptors - گیرنده های inositol 1،4،5-trisphosphateRyanodine receptors - گیرنده های رایانودین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Direct coupling between arachidonic acid-induced Ca2+ release and Ca2+ entry in HEK293 cells Direct coupling between arachidonic acid-induced Ca2+ release and Ca2+ entry in HEK293 cells](/preview/png/9894100.png)
چکیده انگلیسی
Arachidonic acid (AA) modulates intracellular Ca2+ signaling via Ca2+ release or/and Ca2+ entry. However, the mechanism underlies either process is unknown; nor is it clear as to whether the two processes are mechanistically linked. By using Fura2/AM, we found that AA induced mobilization of internal Ca2+ store and an increment in Ca2+, Mn2+ and Ba2+ influx in HEK293 cells. The AA-mediated Ca2+ signaling was not due to AA metabolites, and insensitive to capacitative Ca2+ entry inhibitors. Interestingly, isotetrandrine and Gd3+ inhibited both AA-induced Ca2+ release and Ca2+ entry in a concentration-dependent manner without affecting Ca2+ discharge caused by carbachol, caffeine, or thapsigargin. Additionally, similar pattern of inhibition was observed with tetracaine treatment. More importantly, the three compounds exhibited almost equal potent inhibition of AA-initiated Ca2+ release as well as Ca2+ influx. Therefore, this study, for the first time, provides evidence for a direct coupling between AA-mediated Ca2+ release and Ca2+ entry.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Prostaglandins & Other Lipid Mediators - Volume 75, Issues 1â4, January 2005, Pages 141-151
Journal: Prostaglandins & Other Lipid Mediators - Volume 75, Issues 1â4, January 2005, Pages 141-151
نویسندگان
Dali Luo, Hongli Sun, Xiaomei Lan, RuiPing Xiao, Qide Han,