A glucanase-driven fractionation allows redefinition of Schizosaccharomyces pombe cell wall composition and structure: assignment of diglucan Fulltext Access 11 Pages 2005
Functional RNA microarrays for high-throughput screening of antiprotein aptamers Fulltext Access 11 Pages 2005
Fundamental differences in bioaffinity of amino acid dehydrogenases for N6- and S6-linked immobilized cofactors using kinetic-based enzyme-capture strategies Fulltext Access 11 Pages 2005
The use of prokaryotic DNA methyltransferases as experimental and analytical tools in modern biology Fulltext Access 11 Pages 2005
Solubilization, stabilization, and purification of chemokine receptors using biosensor technology Fulltext Access 11 Pages 2005
Development and evaluation of real competitive PCR for high-throughput quantitative applications Fulltext Access 11 Pages 2005
Förster resonance energy transfer in liposomes: Measurements of transmembrane helix dimerization in the native bilayer environment Fulltext Access 11 Pages 2005
Characterization of oxidized nicotinamide adenine dinucleotide (NAD+) analogues using a high-pressure-liquid-chromatography-based NAD+-glycohydrolase assay and comparison with fluorescence-based measurements Fulltext Access 11 Pages 2005
Comparison of SYTO9 and SYBR Green I for real-time polymerase chain reaction and investigation of the effect of dye concentration on amplification and DNA melting curve analysis Fulltext Access 11 Pages 2005
Sensitivity enhancement of surface plasmon resonance biosensing of small molecules Fulltext Access 11 Pages 2005
Slow irreversible unfolding of Pyrococcus furiosus triosephosphate isomerase: Separation and quantitation of conformers through a novel electrophoretic approach Fulltext Access 11 Pages 2005
Characterization and quantification of C-polysaccharide in Streptococcus pneumoniae capsular polysaccharide preparations Fulltext Access 11 Pages 2005
Optimization of liver biopsy RNA sampling and use of reference RNA for cDNA microarray analysis Fulltext Access 11 Pages 2005
High-density polymerase-mediated incorporation of fluorochrome-labeled nucleotides Fulltext Access 11 Pages 2005
Kinetic measurements and mechanism determination of Stf0 sulfotransferase using mass spectrometry Fulltext Access 11 Pages 2005
A validated liquid chromatography-mass spectrometry method for the determination of leukotrienes B4 and B5 produced by stimulated human polymorphonuclear leukocytes Fulltext Access 11 Pages 2005
Protein fragmentation via liquid chromatography-quadrupole time-of-flight mass spectrometry: The use of limited sequence information in structural characterization Fulltext Access 11 Pages 2005
A potency assay for a replication incompetent adenovirus type 5 carrying a human fgf-4 gene Fulltext Access 11 Pages 2005
Application of fluorophore-assisted carbohydrate electrophoresis to analysis of disaccharides and oligosaccharides derived from glycosaminoglycans Fulltext Access 11 Pages 2005
Analysis of the structure and composition of individual lipoplex particles by flow fluorometry Fulltext Access 11 Pages 2005
Effects of peroxidase substrates on the Amplex red/peroxidase assay: Antioxidant properties of anthracyclines Fulltext Access 11 Pages 2005
Phosphorimaging detection and quantitation for isotopic ion flux assays Fulltext Access 11 Pages 2005
Ligand binding affinity determined by temperature-dependent circular dichroism: Cyclin-dependent kinase 2 inhibitors Fulltext Access 11 Pages 2005
Mass spectrometric identification and quantification of glycosyl flavonoids, including dihydrochalcones with neutral loss scan mode Fulltext Access 11 Pages 2005
Advancing chemistry and biology through diversity-oriented synthesis of natural product-like libraries Fulltext Access 11 Pages 2005
Starch biosynthesis: the primer nonreducing-end mechanism versus the nonprimer reducing-end two-site insertion mechanism Fulltext Access 11 Pages 2005
Synthesis, surface active and antimicrobial properties of new alkyl 2,6-dideoxy-l-arabino-hexopyranosides Fulltext Access 11 Pages 2005
An unambiguous assignment method by 2D selective-TOCSY-HSQC and selective-TOCSY-DQFCOSY and structural analysis by selective-TOCSY-NOESY experiments of a biantennary undecasaccharide Fulltext Access 11 Pages 2005
A dynamic perspective on the molecular recognition of chitooligosaccharide ligands by hevein domains Fulltext Access 11 Pages 2005
Expression, purification, and enzymatic characterization of the dual specificity mitogen-activated protein kinase phosphatase, MKP-4 Fulltext Access 11 Pages 2005
Rational design of novel diketoacid-containing ferrocene inhibitors of HIV-1 integrase Fulltext Access 11 Pages 2005
Evidence for the formation of Michael adducts from reactions of (E,E)-muconaldehyde with glutathione and other thiols Fulltext Access 11 Pages 2005
Functional significance of Glu-77 and Tyr-137 within the active site of isoaspartyl dipeptidase Fulltext Access 11 Pages 2005
Effects of solvent polarity and solvent viscosity on the fluorescent properties of molecular rotors and related probes Fulltext Access 11 Pages 2005
Wanted: new multicomponent reactions for generating libraries of polycyclic natural products Fulltext Access 11 Pages 2005
Phosphatidylserine decarboxylases as genetic and biochemical tools for studying phospholipid traffic Fulltext Access 11 Pages 2005
Evaluation of two lanthanide complexes for qualitative and quantitative analysis of target proteins via partial least squares analysis Fulltext Access 11 Pages 2005
Measurement of the carbohydrate-binding specificity of lectins by a multiplexed bead-based flow cytometric assay Fulltext Access 11 Pages 2005
Identification of the â1 translational frameshift sites using a liquid chromatography-tandem mass spectrometric approach Fulltext Access 11 Pages 2005
Characteristic mass spectral fragments of the organophosphorus agent FP-biotin and FP-biotinylated peptides from trypsin and bovine albumin (Tyr410) Fulltext Access 11 Pages 2005
Multiplex polymerase chain reaction and ligation detection reaction/universal array technology for the traceability of genetically modified organisms in foods Fulltext Access 11 Pages 2005
Immobilization of α1-acid glycoprotein for chromatographic studies of drug-protein binding Fulltext Access 11 Pages 2005
Quantitative nuclear magnetic resonance analysis and characterization of the derivatized Haemophilus influenzae type b polysaccharide intermediate for PedvaxHIB Fulltext Access 11 Pages 2005
Three-dimensional aqueous-derived biomaterial scaffolds from silk fibroin Fulltext Access 11 Pages 2005
Identification and characterization of syntenin binding protein in the black tiger shrimp Penaeusmonodon Fulltext Access 11 Pages 2005
Immobilization and biocatalysis of chlorophyllase in selected organic solvent systems Fulltext Access 11 Pages 2005
Generation of monoclonal antibodies against chromosomal antigens that have a high sequence similarity between human and mouse Fulltext Access 11 Pages 2005
Choice of the adequate detection time for the accurate evaluation of the efficiency of siRNA-induced gene silencing Fulltext Access 11 Pages 2005
Dynamics of single cell property distributions in Chinese hamster ovary cell cultures monitored and controlled with automated flow cytometry Fulltext Access 11 Pages 2005
Synthesis of two bidesmosidic ursolic acid saponins bearing a 2,3-branched trisaccharide residue Fulltext Access 11 Pages 2005
Synthesis and antibacterial activity of new N-linked 5-triazolylmethyl oxazolidinones Fulltext Access 11 Pages 2005
Synthetic modification of the 2-oxypropionic acid moiety in 2-{4-[(7-chloro-2-quinoxalinyl)oxy]phenoxy}propionic acid (XK469), and consequent antitumor effects. Part 4 Fulltext Access 11 Pages 2005
Lobelane analogues as novel ligands for the vesicular monoamine transporter-2 Fulltext Access 11 Pages 2005
Synthesis of glycoporphyrin derivatives and their antiviral activity against herpes simplex virus types 1 and 2 Fulltext Access 11 Pages 2005
Methyllycaconitine analogues have mixed antagonist effects at nicotinic acetylcholine receptors Fulltext Access 11 Pages 2005
Triple layered rotavirus VLP production: Kinetics of vector replication, mRNA stability and recombinant protein production Fulltext Access 11 Pages 2005
Knoop hardness of ten resin composites irradiated with high-power LED and quartz-tungsten-halogen lights Fulltext Access 11 Pages 2005
Stable immobilization of rat hepatocyte spheroids on galactosylated nanofiber scaffold Fulltext Access 11 Pages 2005
Effects of scaffold composition and architecture on human nasal chondrocyte redifferentiation and cartilaginous matrix deposition Fulltext Access 11 Pages 2005
Biosynthetic hydrogel scaffolds made from fibrinogen and polyethylene glycol for 3D cell cultures Fulltext Access 11 Pages 2005
Controlling alginate gel degradation utilizing partial oxidation and bimodal molecular weight distribution Fulltext Access 11 Pages 2005
Radio frequency (rf) plasma spheroidized HA powders: powder characterization and spark plasma sintering behavior Fulltext Access 11 Pages 2005
Liposome-encapsulated actin–hemoglobin (LEAcHb) artificial blood substitutes Fulltext Access 11 Pages 2005
3D microenvironment as essential element for osteoinduction by biomaterials Fulltext Access 11 Pages 2005
Influence of macroporous protein scaffolds on bone tissue engineering from bone marrow stem cells Fulltext Access 11 Pages 2005
Efficient lactic acid production from high salt containing dairy by-products by Lactobacillus salivarius ssp. salicinius with pre-treatment by proteolytic microorganisms Fulltext Access 11 Pages 2005
A direct fluorescence-based assay for RGS domain GTPase accelerating activity Fulltext Access 11 Pages 2005
Electronic microarray analysis of 16S rDNA amplicons for bacterial detection Fulltext Access 11 Pages 2005
Cloning of Tetrahymena cells in a chemically defined medium is possible in the presence of surfactants or at reduced temperature Fulltext Access 11 Pages 2005
The influence of the chemical composition of cell culture material on the growth and antibody production of hybridoma cells Fulltext Access 11 Pages 2005
One-step purification and structural characterization of a recombinant His-tag 11S globulin expressed in transgenic tobacco Fulltext Access 11 Pages 2005
Polycyclic aromatic hydrocarbon biodegradation in extracellular fluids and static batch cultures of selected sub-tropical white rot fungi Fulltext Access 11 Pages 2005
Comparison of different fungal enzymes for bleaching high-quality paper pulps Fulltext Access 11 Pages 2005
Separation and purification of benzylpenicillin produced by fermentation using coupled ultrafiltration and nanofiltration technologies Fulltext Access 11 Pages 2005
Investigations of the transfructosylation reaction by fructosyltransferase from B. subtilis NCIMB 11871 for the synthesis of the sucrose analogue galactosyl-fructoside Fulltext Access 11 Pages 2005
Possibilities for controlling a PHB accumulation process using various analytical methods Fulltext Access 11 Pages 2005
In vitro cytotoxicity of nitric oxide-releasing sol–gel derived materials Fulltext Access 11 Pages 2005
Effect of different levels of NADH availability on metabolic fluxes of Escherichia coli chemostat cultures in defined medium Fulltext Access 11 Pages 2005
Encapsulating live cells with water-soluble chitosan in physiological conditions Fulltext Access 11 Pages 2005
In vitro expression, monoclonal antibody and bioactivity for capsid protein of porcine circovirus type II without nuclear localization signal Fulltext Access 11 Pages 2005
Acetate production from lactose by Clostridium thermolacticum and hydrogen-scavenging microorganisms in continuous culture-Effect of hydrogen partial pressure Fulltext Access 11 Pages 2005
High level expression of functionally active human lactoferrin in silkworm larvae Fulltext Access 11 Pages 2005
Metalloproteinase activity is the sole factor responsible for the growth-promoting effect of conditioned medium in Trichoplusia ni insect cell cultures Fulltext Access 11 Pages 2005
Development of an enzyme-linked immunoreceptor assay (ELIRA) for quantification of the biological activity of recombinant human bone morphogenetic protein-2 Fulltext Access 11 Pages 2005
Feasibility of using ultrasonic irradiation to recover active biomass from waste activated sludge Fulltext Access 11 Pages 2005
Expression and optical properties of green fluorescent protein expressed in different cellular environments Fulltext Access 11 Pages 2005
DNA microarray analysis of the nitrogen starvation response of Corynebacterium glutamicum Fulltext Access 11 Pages 2005
A fast and flexible PEG-mediated transient expression system in plants for high level expression of secreted recombinant proteins Fulltext Access 11 Pages 2005