کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10235183 | 45015 | 2015 | 18 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
pH stabilization of lactic acid fermentation via the glutamate decarboxylation reaction: Simultaneous production of lactic acid and γ-aminobutyric acid
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Neutralization of H+ enhances the substrate conversion rate and affords high product concentrations during lactic acid fermentation. Glutamate decarboxylase (GAD) consumes H+ and catalyzes the α-decarboxylation of glutamate to generate neutral γ-aminobutyric acid (GABA). In this study, the glutamate decarboxylation reaction, mediated by immobilized GAD, was applied to consume the H+ ions produced during lactic acid fermentation by Rhizopus oryzae As3.819. The glutamate decarboxylation reaction could neutralize the H+ produced during fermentation, thereby stabilizing the pH during lactic acid production, while lactic acid fermentation provided H+ for GABA production. By coupling the two biochemical processes (pH 4.7-5.0), a lactic acid concentration of 80.2 g/L with a yield of 67% from glucose and a GABA concentration of 44.8 g/L with 96.7% conversion from glutamate were obtained. These results indicated that the two biochemical processes could be coupled in a single reactor, thereby enabling the production of lactic acid and GABA simultaneously and economically.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 50, Issue 10, October 2015, Pages 1523-1527
Journal: Process Biochemistry - Volume 50, Issue 10, October 2015, Pages 1523-1527
نویسندگان
Weirui Zhao, Jun Huang, Changjiang Lv, Sheng Hu, Shanjing Yao, Lehe Mei, Yinlin Lei,