Development of a novel Aspergillus uracil deficient expression system and its application in expressing a cold-adapted α-amylase gene from Antarctic fungi Geomyces pannorum

- A different Aspergillus uracil deficient transformation system was developed.
- The pyrF and pyrG in Aspergillus are the two separated genes.
- The fungal cold-adapted amylase was firstly cloned and expressed in Aspergillus.
- The biochemical characterization of recombinant amylase was investigated.