Expression, purification and structural characterization of the type 1-specific ATP binding site of IP3 receptor (IP3R1-ATPA)

- Type 1-specific ATP binding site containing domain of IP3R was prepared.
- Production of IP3R1-ATPA protein was established by chaperone co-expression system.
- IP3R1-ATPA adopts mainly a α-helical structure and is thermostable.
- The structural models of IP3R1-ATPA were calculated and validated.