Real-time measurement and modeling of intraparticle pH gradient formation in immobilized cephalosporin C amidase

► Dynamic reaction-diffusion modeling applied to immobilized cephalosporin C amidase. ► First time model parameter estimation to in situ measured real-time intraparticle pH data. ► Model-based estimates of intrinsic immobilized enzyme activity and effective proton diffusion coefficient. ► Correlation of intraparticle pH and concentration gradients with immobilized enzyme effectiveness. ► Model-based optimization of carrier properties for immobilization design.