Enzymatic hydrolysis of racemic ibuprofen esters using Rhizomucor miehei lipase immobilized on different supports

► Immobilization of Rhizomucor miehei lipase was carried out using various protocols. ► The highest immobilization yield and specific activity were obtained in octyl-RML. ► Immobilization via covalent attachment caused to decrease in activity. ► The highest E values were achieved for hydrolysis of ibuprofen butyl ester. ► Octyl-RML retained 75% of its initial activity after 124 h.