کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10236151 | 45140 | 2005 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel two-stage continuous process for excretive expression of hEGF by recombinant E. coli JM101
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In a continuous two-stage process, the first stage was a three-phase fluidized-bed bioreactor. Using polyurethane foam (PUF) as the cell-immobilization support matrix, the recombinant Eshcherichia coli cells were partially immobilized in the PUF matrix. The free cells were produced by fast regeneration of E. coli cells, and entered the second bioreactor, which was a bubble column, for inducing hEGF expression. By this novel process, each stage could be optimized and hEGF expression was enhanced. The effect of dilution rate on cell growth and hEGF expression was evaluated. The results indicated that the hEGF productivity was proportional to the dilution rate when D<0.2 hâ1, but decreased when D>0.2 hâ1. At D=0.2 hâ1 hEGF productivity reached 31.2 mg(h l)â1 which was much higher than that in batch process.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 40, Issue 1, January 2005, Pages 1-4
Journal: Process Biochemistry - Volume 40, Issue 1, January 2005, Pages 1-4
نویسندگان
Xinai Chen, Zhinan Xu, Peilin Cen, W.K.R. Wong,