Optimization of medium compositions favoring butanol and 1,3-propanediol production from glycerol by Clostridium pasteurianum

► Fermentation of glycerol by Clostridium pasteurianum DSM525. ► Statistical investigation of nutrients affecting butanol and 1,3-PDO production. ► Optimization of media for butanol and 1,3-PDO production via a Box-Behnken design. ► The optimal medium for butanol is significantly different from that for 1,3-PDO.