Single-cell mRNA transfection studies: Delivery, kinetics and statistics by numbers

EGFP expression following transfection using messenger RNA (mRNA), in contrast to delivery of plasmid DNA (pDNA), exhibits distinct single-cell time courses that are independent of cell type and well described by mathematical modeling. The dose-response relation is reproduced by a two-step stochastic model for the quantal delivery of mRNA packed in lipoplexes.273