Development of an efficient bioprocess for turanose production by sucrose isomerisation reaction of amylosucrase

► Turanose, a sucrose isomer, is a potential alternative sweetener in food industry. ► An efficient bioprocess was developed for turanose production using amylosucrase. ► Maximal production yield of turanose was 56.2% under the optimised reaction condition. ► High-purify turanose (94.7%) was separated by using a preparative recycling HPLC. ► Major reaction pathway of amylosucrase shifted by controlling substrate concentration.