Purification and characterisation of a novel protease from Cordyceps sinensis and determination of the cleavage site motifs using oriented peptide library mixtures

► A novel protease from Cordyceps sinensis was purified and characterised. ► Its cleavage site motifs were determined by oriented peptide library mixtures. ► The novel protease has completely new cleavage pattern -/-/-/K+-/-/PK/-.