Urinary mesna and total mesna measurement by high performance liquid chromatography with ultraviolet detection

The assay for mesna and total mesna in urine was proved to be linear over the studied ranges of 0.2-30 and 0.2-800 nmol/ml urine, respectively. The mean recoveries over the calibration ranges were 95.4% for mesna and 99.7% for total mesna. The lower limits of detection and quantitation were 0.1 and 0.2 nmol/ml for both the procedures, respectively. The imprecision did not exceed 8.5%. No interference from endogenous substances was observed.