کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10899447 | 1084376 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Superoxide induces protein oxidation in plasma and TNF-α elevation in macrophage culture: Insights into mechanisms of neurotoxicity following doxorubicin chemotherapy
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کلمات کلیدی
HNEDNPHBCANBTApoA-I4-Hydroxy-2-trans-nonenalDOXLPSBCIPnuclear factor κ-light-chain enhancer of activated B cellsKO2NF-κBO2−•2,4-dinitrophenylhydrazine - 2،4-dinitrophenylhydrazineBSA - BSADMSO - DMSONO• - NO •ROS - ROSHydrogen peroxide - آب اکسیژنهbovine serum albumin - آلبومین سرم گاوALP - آلکالن فسفاتازApolipoprotein A-I - آپولیپوپروتئین A-Ibicinchoninic acid - بیسینکنینیک اسیدNitro blue tetrazolium chloride - تترازولیم کلرید نیترو آبیtumor necrosis factor-alpha - تومور نکروز عامل آلفاDoxorubicin - دوکسوروبیسینDimethyl sulfoxide - دیمتیل سولفواکسیدHydroxyl radical - رادیکال هیدروکسیلSOD - سدBlood–brain barrier - سد خونی مغزیBBB - سد خونی مغزیSuperoxide - سوپر اکسیدSuperoxide dismutase - سوکسوکس دیسموتازCancer chemotherapy - شیمی درمانی سرطانTNF-α - فاکتور نکروز توموری آلفاlipopolysaccharide - لیپوپلی ساکاریدMacrophage - ماکروفاژ wild-type - نوع وحشیNitric oxide - نیتریک اکسیدH2O2 - هیدروژن پراکسیدprotein carbonyl - کربونیل پروتئینReactive oxygen species - گونههای فعال اکسیژن•OH - • OH
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Chemotherapy-induced cognitive impairment (CICI) is a quality of life-altering consequence of chemotherapy experienced by a large percentage of cancer survivors. Approximately half of FDA-approved anti-cancer drugs are known to produce ROS. Doxorubicin (Dox), a prototypical ROS-generating chemotherapeutic agent, generates superoxide (O2â
- ) via redox cycling. Our group previously demonstrated that Dox, which does not cross the BBB, induced oxidative damage to plasma proteins leading to TNF-α elevation in the periphery and, subsequently, in brain following cancer chemotherapy. We hypothesize that such processes play a central role in CICI. The current study tested the notion that O2â
- is involved and likely responsible for Dox-induced plasma protein oxidation and TNF-α release. Addition of O2â
- as the potassium salt (KO2) to plasma resulted in significantly increased oxidative damage to proteins, indexed by protein carbonyl (PC) and protein-bound HNE levels. We then adapted this protocol for use in cell culture. Incubation of J774A.1 macrophage culture using this KO2-18crown6 protocol with 1 and 10âµM KO2 resulted in dramatically increased levels of TNF-α produced. These findings, together with our prior results, provide strong evidence that O2â
- and its resulting reactive species are critically involved in Dox-induced plasma protein oxidation and TNF-α release.
- ) via redox cycling. Our group previously demonstrated that Dox, which does not cross the BBB, induced oxidative damage to plasma proteins leading to TNF-α elevation in the periphery and, subsequently, in brain following cancer chemotherapy. We hypothesize that such processes play a central role in CICI. The current study tested the notion that O2â
- is involved and likely responsible for Dox-induced plasma protein oxidation and TNF-α release. Addition of O2â
- as the potassium salt (KO2) to plasma resulted in significantly increased oxidative damage to proteins, indexed by protein carbonyl (PC) and protein-bound HNE levels. We then adapted this protocol for use in cell culture. Incubation of J774A.1 macrophage culture using this KO2-18crown6 protocol with 1 and 10âµM KO2 resulted in dramatically increased levels of TNF-α produced. These findings, together with our prior results, provide strong evidence that O2â
- and its resulting reactive species are critically involved in Dox-induced plasma protein oxidation and TNF-α release.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Letters - Volume 367, Issue 2, 28 October 2015, Pages 157-161
Journal: Cancer Letters - Volume 367, Issue 2, 28 October 2015, Pages 157-161
نویسندگان
Jeriel T.R. Keeney, Sumitra Miriyala, Teresa Noel, Jeffrey A. Moscow, Daret K. St. Clair, D. Allan Butterfield,