کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11027408 | 1666296 | 2019 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quantification of aflatoxin B1 in vegetable oils using low temperature clean-up followed by immuno-magnetic solid phase extraction
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In the present study, a convenient and highly efficient method was developed to quantify aflatoxin B1 (AFB1) in oil samples. Low temperature clean-up (LTC) followed by immuno magnetic solid phase extraction (IMSPE) was used to clean up oil samples. LTC assisted in freezing out the interference from the oil matrix while IMSPE further helped to preconcentrate the targeted analyte. For IMSPE, we synthesised and characterised anti-AFB1 monoclonal antibody-functionalised magnetic nanoparticles. Oil sample extraction was then carried out using LTC-IMSPE. The proposed method showed satisfactory efficiency and reproducibility with recovery rates being within the range of 79.6-117.9%, with a relative standard deviation below 11.48%. The sensitivity of the method was satisfactory with the limits of detection and quantification being as low as 0.0048 and 0.0126â¯ng·gâ1, respectively. Real sample analysis was carried out for five kinds of different vegetable oils. The results suggest that the method developed is very sensitive and accurate.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 275, 1 March 2019, Pages 390-396
Journal: Food Chemistry - Volume 275, 1 March 2019, Pages 390-396
نویسندگان
Xi Yu, Zhanming Li, Mengzhe Zhao, Siew Cheng Stella Lau, Hui Ru Tan, Wei Jie Teh, Hongshun Yang, Chao Zheng, Yuquan Zhang,