کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
1172692 961532 2016 5 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
Enzymatic cycling method using creatine kinase to measure creatine by real-time detection
ترجمه فارسی عنوان
روش چرخه آنزیمی با استفاده از کراتین کیناز برای اندازه گیری کراتین با تشخیص در زمان واقعی
کلمات کلیدی
چرخه آنزیمی؛ کراتین کیناز؛ کراتین؛ ADP وابسته به گلوکوکیناز؛ تشخیص زمان واقعی CK، کراتین کیناز؛ CP، کراتین فسفات؛ PK، پیرووات کیناز؛ LDH، لاکتات دهيدروژناز؛ 3α-HSD، 3α-hydroxysteroid dehydrogenase؛ dADP، deoxyadenosine diphosp
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی

We have developed a novel enzymatic cycling method that uses creatine kinase (CK) to measure creatine. The method takes advantage of the reversibility of the CK reaction in which the forward (creatine phosphate forming) and reverse reactions are catalyzed in the presence of an excess amount of ATP and IDP, respectively. Real-time detection was accomplished using ADP-dependent glucokinase (ADP–GK) together with glucose-6-phosphate dehydrogenase. ADP, one of the cycling reaction products, was distinguished from IDP by using the nucleotide selectivity of the ADP–GK. The increasing level of ADP was measured from the level of reduced NADP at 340 nm. The method is appropriate for an assay that requires high sensitivity because the rate of increase in absorbance at 340 nm is proportional to the amount of CK present in the reaction mix. We reasoned that the method with CK in combination with creatinine amidohydrolase could be used to assay creatinine, an important marker of kidney function. Our results confirmed the quantitative capability of the assay.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 506, 1 August 2016, Pages 8–12
نویسندگان
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