کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
1172684 961527 2016 5 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
Exciton circular dichroism couplet arising from nitrile-derivatized aromatic residues as a structural probe of proteins
ترجمه فارسی عنوان
زوج دی کرومیک دایره ای اکسیتون ناشی از بقایای آروماتیک مشتق شده از نیترولی به عنوان یک پروب ساختاری پروتئین
کلمات کلیدی
طیف سنجی سی دی؛ کوپلینگ اکسیته؛ 5-Cyanotryptophan؛ p-Cyanophenylalanine؛ ساختار پروتئین CD، دی کروم دایره ای؛ UV، اشعه ماوراء بنفش؛ Trp، تریپتوفان؛ TrpCN، 5-cyanotryptophan؛ PheCN، p-سینوفنیل آلانین؛ IR، مادون قرمز؛ HPLC، کروم مایع با کارایی بالا
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی

Exciton coupling between two chromophores can produce a circular dichroism (CD) couplet that depends on their separation distance, among other factors. Therefore, exciton CD signals arising from aromatic sidechains, especially those of tryptophan (Trp), have been used in various protein conformational studies. However, the long-wavelength component of the commonly used CD couplet produced by a pair of Trp residues is typically located around 230 nm, thereby overlapping significantly with the protein backbone CD signal. This overlap often prevents a direct and quantitative assessment of the Trp CD couplet in question without further spectral analysis. Here, we show that this inconvenience can be alleviated by using a derivative of Trp, 5-cyanotryptophan (TrpCN), as the chromophore. Specifically, through studying a series of peptides that fold into either α-helical or ß-hairpin conformations, we demonstrate that in comparison with the Trp CD couplet, that arising from two TrpCN residues not only is significantly red-shifted but also becomes more intense due to the larger extinction coefficient of the underlying electronic transition. In addition, we show that a pair of p-cyanophenylalanines (PheCN) or a PheCN–TrpCN pair can also produce a distinct exciton CD couplet that can be useful in monitoring conformational changes in proteins.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 507, 15 August 2016, Pages 74–78
نویسندگان
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