کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1185833 | 963414 | 2009 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Purification and characterization of chymotrypsins from the hepatopancreas of crucian carp (Carassius auratus)
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Purification and characterization of chymotrypsins from the hepatopancreas of crucian carp (Carassius auratus) Purification and characterization of chymotrypsins from the hepatopancreas of crucian carp (Carassius auratus)](/preview/png/1185833.png)
چکیده انگلیسی
Two chymotrypsins (chymotrypsin A and B) have been purified to homogeneity from the hepatopancreas of crucian carp (Carassius auratus) by ammonium sulphate fractionation and chromatographies on DEAE-Sepharose, Sephacryl S-200 HR, Phenyl-Sepharose and SP-Sepharose. The molecular masses of chymotrypsin A and B were approximately 28 and 27 kDa, respectively, by SDS-PAGE. Purified chymotrypsins also revealed single bands by native-PAGE. Optimum temperatures of chymotrypsin A and B were 40 and 50 °C, and optimal pHs were 7.5 and 8.0 using Suc-Leu-Leu-Val-Tyr-AMC as substrate. Both enzymes were effectively inhibited by serine proteinase inhibitors and slightly activated by metal ions such as Ca2+ and Mg2+, while inactivated by Mn2+, Cd2+, Cu2+, Fe2+ to different degrees. Apparent Kms of chymotrypsin A and B were 1.4 and 0.5 μM, and Kcats were 2.7 Sâ1 and 3.4 Sâ1, respectively. Immunoblotting analysis using anti-chymotrypsin B weakly cross reacted with chymotrypsin A.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 116, Issue 4, 15 October 2009, Pages 860-866
Journal: Food Chemistry - Volume 116, Issue 4, 15 October 2009, Pages 860-866
نویسندگان
Feng Yang, Wen-Jin Su, Bao-Ju Lu, Tao Wu, Le-Chang Sun, Kenji Hara, Min-Jie Cao,