β-Cyclodextrin facilitates simultaneous analysis of six bioactive components in Rhizoma Smilacis Glabrae by capillary zone electrophoresis

A cyclodextrin-modified capillary zone electrophoretic method was developed for the separation and determination of trans-resveratrol, astilbin, taxifolin, shikimic acid, syringic acid and ferulic acid in Rhizoma Smilacis Glabrae. A running buffer comprising 20 mM borax and 2 mM β-cyclodextrin at pH 9.46 was used. These six components were well separated from each other within 8 min at a voltage of 25 kV, temperature of 25 °C and detection wavelength of 214 nm. The relative standard deviations of migration time ranged from 0.07% to 0.30% while those of the peak area ratios ranged from 2.66% to 4.12% for six determinations of analytes at the concentration of 5 and 25 μg mL−1. The correlation coefficients of the calibration curves of analytes were all >0.9990, and the recoveries were from 97.5% to 108.3%. The method was successfully applied to determine the bioactive components in 12 samples of Rhizoma Smilacis Glabrae collected from China.