Occurrence of cyanogenic glycoside and cyanide in the Malaysian rubber seed oil

The cyanogenic glycoside (linamarin) in rubber seed oil (RSO) extracted using different solvents such as hexane (RSOh), a mixture of chloroform and methanol (RSOchl+mth) and ethanol (RSOeth) was also studied. Colorimetric method was carried out to determine the presence of such compounds. Toxicological test using rats was also conducted to further confirm the absence of such compounds. The determination of cyanide by using colorimetric method was demonstrated by no response of the cyanide in RSO and did not show any color comparing with commercial cyanide which observed a blue color. Rubber seed oil did not show any toxic potential to the rats. This can be attributed to the absence of hazardous linamarin in RSO.