کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
19671 43116 2016 5 صفحه PDF ندارد دانلود کنید
عنوان انگلیسی مقاله
Multiplex PCR for the early detection of fumonisin producing Fusarium verticillioides
ترجمه فارسی عنوان
Multiplex PCR برای تشخیص زودهنگام فومونیسین تولید کننده Fusarium verticillioides
کلمات کلیدی
فوزاریوم؛ غلات؛ ذرت؛ پدي؛ سورگوم؛ اسپایکینگ؛ میوکوتوکسین
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی

In the present study, fumonisin producing Fusarium verticillioides was specifically detected in pure cultures, cereal samples and plant materials by multiplex PCR using one forward VERTF-1 and two reverse primers VERTR and VERTF-2. A total of 326 Fusarium isolates were obtained from maize, sorghum, paddy wheat and pearl millet samples collected from different districts of Karnataka, India. All Fusarium species were subjected to single round of PCR with species specific and fumonisin specific primers which recorded 59.50% of F. verticillioides and 53.98% of fumonisin producing F. verticillioides. Maize samples recorded highest frequency 34.42% of fumonisin producing F. verticillioides followed by paddy 28.57% and sorghum 16.66%. Sensitivity of multiplex PCR experiment was conducted by whole grain experiment of the collected cereals, roots and leaves of the cereal samples by diluting the DNA 10 to 100 times in which 1:50, 1:75 and 1:100 diluted samples recorded positive. The developed multiplex PCR assay provided a powerful tool for the accurate detection, identification and discrimination of potential fumonisin producing F. verticillioides strains among the population. The present study is the first report of developing the multiplex PCR method for early detection of fumonisin producing F. verticillioides from cereal samples, pure cultures and plant parts.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Bioscience - Volume 13, 1 March 2016, Pages 84–88
نویسندگان
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