کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2115493 | 1084597 | 2006 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quantitative analysis of aromatase, sulfatase and 17β-HSD1 mRNA expression in soft tissue metastases of breast cancer
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Expression of the estrogen-synthesizing genes aromatase, steroid sulfatase (STS) and 17β-hydroxysteroid dehydrogenase type1 (17β-HSD1) has been shown to be up-regulated in primary breast cancer tissue but their expression status in metastatic tumor tissue has yet to be determined. The mRNA expression levels of the three estrogen-synthesizing genes as well as of tumor necrosis factor (TNF)-α, interleukin (IL)-6 and cyclooxygenase (COX)-2, all of which have been reported to up-regulate the estrogen-synthesizing genes, were determined by means of a real-time PCR assay in 100 primary breast cancer tissues and 15 soft tissue metastases. In addition, PCR-gel electrophoresis was used to determine the proportion (%) of promoter (l.4, l.3, Pll and l.7) usage of aromatase. Aromatase and STS mRNA levels were significantly (P=0.04 and P=0.03, respectively) higher in soft tissue metastases than in primary tumors, while 17β-HSD1 mRNA levels tended (P=0.09) to be higher. The proportions of the promoter usages were very similar for primary tumors and soft tissue metastases, and the mRNA levels of TNF-α, IL-6 and COX-2 were not significantly different. Levels of aromatase, STS and 17β-HSD1 mRNA are up-regulated in soft tissue metastases compared to those in primary tumors, suggesting that intra-tumoral estrogen synthesis may play a significant role in the growth stimulation of tumor cells in soft tissue metastases as in primary tumors. TNF-α, IL-6 and COX-2, on the other hand, are unlikely to be implicated in this up-regulation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Letters - Volume 243, Issue 1, 8 November 2006, Pages 23-31
Journal: Cancer Letters - Volume 243, Issue 1, 8 November 2006, Pages 23-31
نویسندگان
Natsumi Irahara, Yasuo Miyoshi, Tetsuya Taguchi, Yasuhiro Tamaki, Shinzaburo Noguchi,