کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2585745 1130879 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vivo direct patulin-induced fluidization of the plasma membrane of fission yeast Schizosaccharomyces pombe
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
In vivo direct patulin-induced fluidization of the plasma membrane of fission yeast Schizosaccharomyces pombe
چکیده انگلیسی

Patulin is a toxic metabolite produced by various species of Penicillium, Aspergillus and Byssochlamys. In the present study, its effects on the plasma membrane of fission yeast Schizosaccharomyces pombe were investigated. The phase-transition temperature (G) of untreated cells, measured by electron paramagnetic resonance spectrometry proved to be 14.1 °C. Treatment of cells for 20 min with 50, 500, or 1000 μM patulin resulted in a decrease of the G value of the plasma membrane to 13.9, 10.1 or 8.7 °C, respectively. This change in the transition temperature was accompanied by the loss of compounds absorbing light at 260 nm. Treatment of cells with 50, 500 or 1000 μM patulin for 20 min induced the efflux of 25%, 30.5% or 34%, respectively, of these compounds. Besides its cytotoxic effects an adaptation process was observed. This is the first study to describe the direct interaction of patulin with the plasma membrane, a process which could definitely contribute to the adverse toxic effects induced by patulin.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 48, Issue 7, July 2010, Pages 1898–1904
نویسندگان
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