کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4337704 | 1614808 | 2014 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
MicroRNA-132 dysregulation in Toxoplasma gondii infection has implications for dopamine signaling pathway
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کلمات کلیدی
COMTMouse striatumMAOBDRD5MAOAPP1Dopamine receptor D13-MTTLRIPA5-HIAADRD1DOPACDPIDARPP-32pKaPBSLPSCatechol-O-methyltransferaseqPCR5-HT3-methoxytyramine - 3-methoxythyramine3,4-dihydroxyphenylacetic acid - 3،4-دی هیدروکسی فنیل اسیدهای اسید5-Hydroxyindoleacetic acid - 5-هیدروکسی سدیم اسیدBDNF - BDNF یا فاکتور نورونزایی مشتقشده از مغز Homovanilic acid - اسید هومیانیلیکmiR-132 - به miR-132Ingenuity Pathway Analysis - تجزیه و تحلیل راه IngenuityToxoplasma gondii - توکسوپلاسما گوندیToll-like receptor - تیالآرdays post infection - روز پس از عفونتSerotonin - سروتونینbrain derived neurotrophic factor - عامل مغز استخوان مغز استخوان استLipopolysaccharides - لیپوپلی ساکارید هاPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریmonoamine oxidase A - مونوآمین اکسیداز Amonoamine oxidase B - مونوآمین اکسیداز BMicroRNA - میکرو RNA MiRNA - میکروRNA، ریزآرانای، miRNAquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیprotein phosphatase 1 - پروتئین فسفاتاز 1protein kinase A - پروتئین کیناز AHVA - چهhigh-performance liquid chromatography - کروماتوگرافی مایعی کاراHPLC - کروماتوگرافی مایعی کاراquality control - کنترل کیفیت
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
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چکیده انگلیسی
Congenital toxoplasmosis and toxoplasmic encephalitis can be associated with severe neuropsychiatric symptoms. However, which host cell processes are regulated and how Toxoplasma gondii affects these changes remain unclear. MicroRNAs (miRNAs) are small noncoding RNA sequences critical to neurodevelopment and adult neuronal processes by coordinating the activity of multiple genes within biological networks. We examined the expression of over 1000 miRNAs in human neuroepithelioma cells in response to infection with Toxoplasma. MiR-132, a cyclic AMP-responsive element binding (CREB)-regulated miRNA, was the only miRNA that was substantially upregulated by all three prototype Toxoplasma strains. The increased expression of miR-132 was also documented in mice following infection with Toxoplasma. To identify cellular pathways regulated by miR-132, we performed target prediction followed by pathway enrichment analysis in the transcriptome of Toxoplasma-infected mice. This led us to identify 20 genes and dopamine receptor signaling was their strongest associated pathway. We then examined myriad aspects of the dopamine pathway in the striatum of Toxoplasma-infected mice 5Â days after infection. Here we report decreased expression of D1-like dopamine receptors (DRD1, DRD5), metabolizing enzyme (MAOA) and intracellular proteins associated with the transduction of dopamine-mediated signaling (DARPP-32 phosphorylation at Thr34 and Ser97). Increased concentrations of dopamine and its metabolites, serotonin (5-HT) and 5-hydroxyindoleacetic acid were documented by HPLC analysis; however, the metabolism of dopamine was decreased and 5-HT metabolism was unchanged. Our data show that miR-132 is upregulated following infection with Toxoplasma and is associated with changes in dopamine receptor signaling. Our findings provide a possible mechanism for how the parasite contributes to the neuropathology of infection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 268, 30 May 2014, Pages 128-138
Journal: Neuroscience - Volume 268, 30 May 2014, Pages 128-138
نویسندگان
J. Xiao, Y. Li, E. Prandovszky, S.S. Karuppagounder, C.C. Jr., V.L. Dawson, T.M. Dawson, R.H. Yolken,