کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5132438 1492052 2018 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Lipase from liver of seabass (Lates calcarifer): Characteristics and the use for defatting of fish skin
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Lipase from liver of seabass (Lates calcarifer): Characteristics and the use for defatting of fish skin
چکیده انگلیسی


- Lipase from liver of seabass was purified using a series of chromatographies.
- Lipase from liver of seabass had a MW of 60 kDa.
- Lipase removed the lipids from seabass skin in a dose dependent manner.
- Lipase showed higher efficacy than isopropanol in defatting of seabass skin.

Lipase from liver of seabass (Lates calcarifer), with a molecular weight of 60 kDa, was purified to homogeneity using ammonium sulfate precipitation and a series of chromatographies, including diethylaminoethyl sepharose (DEAE) and Sephadex G-75 size exclusion columns. The optimal pH and temperature were 8.0 and 50 °C, respectively. Purified lipase had Michaelis-Menten constant (Km) and catalytic constant (kcat) of 0.30 mM and 2.16 s−1, respectively, when p-nitrophenyl palmitate (p-NPP) was used as the substrate. When seabass skin was treated with crude lipase from seabass liver at various levels (0.15 and 0.30 units/g dry skin) for 1-3 h at 30 °C, the skin treated with lipase at 0.30 units/g dry skin for 3 h had the highest lipid removal (84.57%) with lower lipid distribution in skin. Efficacy in defatting was higher than when isopropanol was used. Thus, lipase from liver of seabass could be used to remove fat in fish skin.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 240, 1 February 2018, Pages 9-15
نویسندگان
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